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four. When the peaks for fluoxetine and protriptyline are settled insufficiently, how might you change the mobile phase to enhance their separation?There are numerous chromatographic modes of separation and every has its personal merits. Provided underneath is usually a HPLC column variety tree to manual viewers to select the proper mode of analysi

Ion pair reagents are required to be a mobile-phase additive when structurally or chemically or polarity wise inseparable closely related compounds are to get divided [21, 22]. By way of example, if a mixture of ionic and nonionic analyte(s) obtaining the identical polarity and very same retention time is required to be divided, begin by optimizing